Advanced Imaging Techniques for Neuroscience

Progress in microscopy has a long history of triggering major advances in neuroscience, and it is accelerating on many fronts: morphological and functional labeling, microscope instrumentation and imaging modalities, image processing and data analysis etc. These innovations are potentiating our ability to monitor, measure and manipulate biological structures and activities inside complex and intact nervous system model systems with ever higher throughput, robustness, precision and sensitivity and on ever wider temporal and spatial scales.

This Cajal course will bring together leading developers and practitioners of cutting-edge imaging techniques that push the frontiers of neuroscience research. The course follows up on previous Cajal school editions on this topic. The course will cover a broad spectrum of concepts and practical techniques, both classic and new, to provide a solid basis and critical guidance for newcomers and experienced users alike, wishing to pick up skills and learn about new developments and avenues in microscopy and its impact on neuroscience.

The applications will span a wide spectrum of neurobiological topics and preparations in an exemplary fashion, from brain development, plasticity and neuro-immune system interactions in cell cultures, brain slices and in vivo using the mouse and zebrafish brain as main model systems.

A series of pedagogical lectures and seminars will be complemented by hands-on practical training in small groups using experimental setups and tools provided by the Bordeaux School of Neuroscience, the Bordeaux Imaging Center, the course faculty & instructors, including a number of leading research labs on Bordeaux Neurocampus, as well as brand-new demo equipment from microscope manufacturers.

Course directors

  • Valentin Nägerl (Bordeaux University, France)
  • Francesca Odoardi (Göttingen University, Germany)
  • Jan Huisken (Göttingen University, Germany)

Overall, the program is split into these broad complementary categories:

  • Multi-scale imaging of fixed brain tissue: from EM and super-resolution imaging to cleared tissue imaging
  • From in vitro to in vivo imaging in brain preparations
  • Functional neuronal imaging in behaving organisms

The course is divided into two 9-day long experimental blocks, offering each 6 to 8 experimental projects, where teams of 2 to 3 students pick projects for both blocks based on interest and compatibility.

The main goals of the course are to:

  1. Teach students the theoretical foundation of advanced imaging techniques for molecular, cellular and systems neuroscience research.
  2. Enable them to design and carry out frontier imaging experiments, analyze the results and present them to the other students and faculty at the end.
  3. Allow them to gain enough relevant experience and build a knowledge base & network (with peers, faculty and companies) about the approaches to be able to establish them in their labs upon return.

This course offers experimental projects in these concrete areas:

  • Nanoscale monitoring and manipulation of the dynamic molecular organization of synapses using STED, SMLM and EM approaches.
  • Functional imaging of neuronal and astroglial cellular and network activity using modern optical biosensors for calcium (intra- and extracellular), synaptic glutamate, dopamine and other signaling molecules.
  • Shadow imaging of anatomical micro-structures and spaces in brain slices and in vivo using a variety of imaging modalities, including STED, confocal, 2-photon and light sheet microscopy in wildtype and animal models of brain diseases.
  • Super-resolution imaging of the biophysical properties (viscosity, topology) of the extracellular space using single-particle tracking (QD and carbon nanotubes).
  • Whole-brain multi-scale imaging by tissue clearing, expansion microscopy, and light sheet microscopy
  • Fast functional imaging in behaving zebrafish, danionella and other emerging model organisms
  • Imaging and analysis of chemotactic leukocyte migration in 3D environments
  • Functional imaging of calcium imaging in immune cells in vitro and in vivo.
  • Intravital Imaging via two-photon microscopy of immune cell motility in the CNS in healthy and autoimmune conditions

Will be announced soon.