Cancer neuroscience represents an interdisciplinary field at the forefront of cancer research, seamlessly combining insights from neurobiology and oncology. The intricate interplay between the nervous system and cancer has yielded profound insights into the mechanisms of tumor progression and the tumor microenvironment. The latter, comprising neuro-glial, immune, and endothelial networks, has been identified as a critical determinant of cancer growth and metastasis.
Cutting-edge technologies inspired by the neuroscience field are transforming our ability to study brain cancers from a neural perspective. This course will enable students to integrate theoretical and methodological concepts in neuro-oncology, combining modern neuroscience approaches with hands-on experience in state-of-the-art methodologies. The course provides an integrated understanding of the crosstalk between neuro-oncology and neuroscience.
Course directors
- Antonio C. Pagano Zottola (Bordeaux Institute of Oncology – INSERM, France)
- Anna Golebiewska (Luxembourg Institute of Health, Luxembourg)
- Varun Venkataramani (German Cancer Research Center, Germany)
Maialen Arrieta, Institut de Biochimie et Génétique Cellulaires, France
Chiara Bastiancich, Institut de Neurophysiopathologie, France
Luigi Bellocchio, Neurocentre Magendie, Bordeaux University, France.
Doriane Bomont, Institut de Biochimie et Génétique Cellulaires, France
Clementine Bosch Bouju, NutriNeuro, Bordeaux University, France
Kevin Boyé, Paris Cardiovascular Research Center, France
Lucie Brisson, Bordeaux Institute of Oncology, France
Audrey Burban, Institut de Biochimie et Génétique Cellulaires, France
Ahmad Charanek, Bordeaux Institute of Oncology, France
Thomas Daubon, Institut de Biochimie et Génétique Cellulaires, France
Rémi Galland, IINS, Bordeaux University, France
Emanuelle Georget, Bordeaux Institute of Oncology
Nassim Haffiane, IINS, Bordeaux University, France
Maria Haykal, Institut de Biochimie et Génétique Cellulaires, France
Camille Humeau, Bordeaux Institute of Oncology, France
Teo Leboucq, Bordeaux Institute of Oncology, France
Valentina Lopardo, Università di Salerno, Italy
Oceane Martin, Institut de Biochimie et Génétique Cellulaires, France
Thomas Mathivet, Bordeaux Institute of Oncology, France
Pilar Moreno-Sanchez, Luxembourg Institute of Health, Luxembourg
Clement Morgat, INCIA, Bordeaux University, France
Ekin Reyhan, Universität Heidelberg, Germany
Mahsa Rezaeipour, Luxembourg Institute of Health, Luxembourg
Beatrice Senigagliesi, IINS, Bordeaux University, France
Kirill Smirnov, Institut de Psychiatrie et Neurosciences de Paris, France
Stella Soyka, Universität Heidelberg, Germany
Cloe Tessier, Bordeaux Institute of Oncology
Olivier Thoumine, IINS, Bordeaux University, France
Cultures of brain tumor cells
Primary cultures of neurons, astrocytes, and oligodendrocytes
Primary culture of enteric nervous system cells (ENS) and ex vivo analysis of ENS structure in mouse models
Tumor cell spheroid cultures
Organoids
Organotypic brain slices
Co-culture of tumor cells with brain cells
Culture of glioblastoma cells in 3D-living systems of brain
Stereotaxic surgery for tumor implantation
Tumor debulking via the biopsy punch technique
Live imaging of cell-cell interactions
Confocal microscopy
Spinning-disk microscopy
Super-resolution microscopy
Two-photon microscopy
Multiphoton longitudinal imaging of tumor progression
DREADD manipulation and calcium imaging in mice during reward-based navigation.
Neuronal calcium activity using a head-mounted miniature microscope on animals performing behavioral tasks
Magnetic Resonance Imaging
Radiolabeling with radiometals and characterization of radio pharmaceuticals
SPECT/CT imaging and post-mortem biodistribution
PBMC isolation from human blood
Isolation of murine tumor-infiltrating immune cells
Characterization of immune cells by flow cytometry
High resolution respirometry using Resipher system and Oroboros Oxygraph-2k
Isolation of proteins and exosomes.
PamGene Technology
Methods to induce Blood-brain barrier opening in brain tumor models
Electrophysiology in multielectrode array
Electrophysiological patch-clamp recordings
Introduction to signal preprocessing, spike detection, and activity mapping
Image analysis using Imaris software
Analysis of cell motility with ImageJ
Analysis of spatial transcriptomics datasets using Seurat, Squidpy, R and Python
Analysis of 10x Genomics Visium datasets from human glioblastoma samples
Exploration of 10x Genomics Xenium imaging-based spatial data
Honorary lecture from Brain Prize Winner
Frank Winkler, German Cancer Research Center, Germany.
Keynote Speakers
Thomas Daubon, Institut de Biochimie et Génétique Cellulaires, France.
Manuel Valiente, Centro Nacional de Investigaciones Oncológicas, Spain.
Simona Parrinello, UCL Cancer Institute, UK.
Aurélie Tchoghandjian, Institut de Neurophysiopathologie, France.
Hélène Castel, Laboratoire de Différenciation et Communication Neuronale et Neuroendocrine, France.
Hrvoye Miletic, University of Bergen, Norway.
Vidhya Ravi Madapusi, Freiburg Institute for Advanced Studies, Germany.
Leila Akkari, Netherlands Cancer Institute, Netherlands.
Dieter Henrik Heiland, Department of Neurosurgery, Germany.
Elena Ciaglia, Università di Salerno, Italy.