Advanced imaging techniques for cellular and systems neuroscience #AITCSN

October 18 to November 5 (2021)

Due to the COVID-19 crisis, this course has been rescheduled (initially planned on March 2020)

Rapid advances in live imaging of targeted cellular morphologies and functions underpin the emerging revolution in our understanding of synapses, circuits, and behaviour. In keeping with its long-established tradition, this Cajal course will assemble at its international faculty leading experts in developing and exploiting cutting-edge imaging techniques that have been propelling such advances. How to combine genetically encoded fluorescence labelling with behavioural designs, micro-circuit monitoring, or single-molecule tracking, how to avoid pitfalls of having false-positive observations and inherent noise, how to best analyse your multi-dimensional data will be, among others, the recurrent subjects of the Course. An intense program of lectures and seminars will be combined with supervised, hands-on practical training modules and group assignments using experimental setups and tools provided by the world-leading research environment of the Bordeaux School of Neuroscience.

Course director & co-directors

  • Dmitri Rusakov (London’s Global University, UK)
  • Tatiana Korotkova (Max Planck Institute for Metabolism Research, Germany)
  • Grégory Giannone (Bordeaux Neurocampus, France)

October 18 – 11:00am
Dmitri Rusakov (University College London, UK)
Monitoring variable efficacy of individual synapses with multiplexed imaging

October 20 – 9:00am
Christophe Leterrier (Aix-Marseille University, France)
The axonal cytoskeleton at the nanoscale

October 20 – 11:00am
Olga Garaschuk (University of Tübingen, Germany)
Deciphering the functional properties of mouse and human microglia

October 21 – 9:00am
Kirill Volynski (University College London, UK)
iGluSnFR imaging reveals the differential regulation of synchronous and asynchronous vesicular release at small central synapses

October 21  – 11:00am
Daniel Choquet (Bordeaux Neurocampus, France)
Nanoscale organization and function of excitatory synapses

October 25 – 9:00am
Timothy Ryan (Cornell University, USA)
Support your local synapse: vulnerabilities and control points in synapse function

October 25 – 11:00am
Dragomir Milovanovic (Charité University Clinic, Germany)
Phase separation at the synaptic boutons

October 28 – 9:00am
Tatiana Korotkova (University of Cologne, Germany)
Simple pleasures: neuronal regulation of feeding and social interactions

October 28 – 11:00am
Laurent Groc (Bordeaux Neurocampus, France)
Imaging membrane receptor dynamics: basics and role in neuropsychiatric disorders

October 29 – 9:00am
Andrea Volterra (University of Lausanne, Switzerland)
Use of 1D-to-3D two-photon Ca2+ imaging to decode the language of astrocyte communication

October 29 – 11:00am
Christian Henneberger (University of Bonn, Germany)
Visualizing the extracellular signalling of glutamate receptor ligands

November 2 – 9:00am
Anna Beyeler (Bordeaux Neurocampus, France)
Circuits dissection in emotional valence and anxiety: case studies on the amygdala and insular cortex

November 2 – 11:00am
Valentin Nagerl (Bordeaux Neurocampus, France)
Super-resolution microscopy for neuroscience: from basic principles to recent applications

November 3 – 9:00am
Michael Hausser (University College London, UK)

November 3 – 11:00am
Gregory Giannone (Bordeaux Neurocampus, France)

November 3 – 5:30pm
Thomas Blanpied (University of Maryland, USA)
Dynamic control of synapse nanoarchitecture

Stephane Bancelin (Bordeaux Neurocampus, France)
Francisco de los Santos (Max Planck Institute Cologne, Germany)
Yulia Dembitskaya (Bordeaux Neurocampus, France)
Julien Dupuis (Bordeaux Neurocampus, France)
Christian Hoffman (Charité University Clinic, Germany)
Ani Jose (Bordeaux Neurocampus, France)
Olga Kopach (University College London, UK)
Philipe R. F. Mendonça (University College London, UK)
Dragomir Milovanovic (Charité University Clinic, Germany)
Olivier Nicole (Bordeaux Neurocampus, France)
Jakob Rentsch (Charité Univeristy Clinic, Germany)
Olga Tiurikova (University College London, UK)
Petr Unichenko (University of Bonn, Germany)
Hanna Van Den Munkhof (Max Planck Institute Cologne, Germany)
Kaiyu Zheng (University College London, UK)
Xuesi Zhou (Bordeaux Neurocampus, France)

  • Project 1: Imaging prefrontal cortex neurons encoding social information
  • Project 2: RESOLFT nanoscopy to study the fast reorganization of dendritic spines
  • Project 3: Nanoscale organization and dynamics of synapsin condensates
  • Project 4: Quantitative intracellular calcium measurements using fluorescent lifetime imaging microscopy
  • Project 5: A viral strategy for targeting and manipulating principal neurons: probing the glutamatergic synapse function at multi-synapse imaging approach
  • Project 6: Combining FLIM-FRET and SPT to characterize the functional impact of rare NMDAR variants associated with brain disorders
  • Project 7: High spatial-temporal imaging of a heterogeneous population of synapses in neuronal cultures using the iGluSnFR probe
  • Project 8: Probing of astroglial Ca2+ dynamics in organised brain tissue through fluorescent intensity and lifetime measurements
  • Project 9: Super-resolution shadow imaging in the mouse brain
  • Project 10: Assessing the mechanosensitive properties of the membrane periodic skeleton (MPS) in neurons
  • Project 11: FRET imaging of NMDA receptor co-agonists dynamics
  • Project 12: Imaging hippocampal place cells in freely moving animals
  • Project 13: Identification of structural plasticity of glutamatergic synapses after removal of the brain extracellular matrix (ECM) using 3D-STED microscopy

Keynote speakers

Anna Beyeler (Bordeaux Neurocampus, France)
Thomas Blanpied (University of Maryland School of Medicine, USA)
Daniel Choquet (Bordeaux Neurocampus, France)
Olga Garaschuk (University of Tuebingen, Germany)
Laurent Groc (Bordeaux Neurocampus, France)
Michael Haüsser (University College London, UK)
Christian Henneberger (University of Bonn, Germany)
Christophe Leterrier (Aix-Marseille University, France)
Valentin Nägerl (Bordeaux Neurocampus, France)
Timothy A. Ryan (Weill Cornell Medical College, USA)
Andrea Volterra (University of Lausanne, Switzerland)
Kirill Volynski (University College London, UK)

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