Advanced imaging techniques for cellular and systems neuroscience #AITCSN

October 18 to November 5 (2021)

Due to the COVID-19 crisis, this course has been rescheduled (initially planned on March 2020)

Rapid advances in live imaging of targeted cellular morphologies and functions underpin the emerging revolution in our understanding of synapses, circuits, and behaviour. In keeping with its long-established tradition, this Cajal course will assemble at its international faculty leading experts in developing and exploiting cutting-edge imaging techniques that have been propelling such advances. How to combine genetically encoded fluorescence labelling with behavioural designs, micro-circuit monitoring, or single-molecule tracking, how to avoid pitfalls of having false-positive observations and inherent noise, how to best analyse your multi-dimensional data will be, among others, the recurrent subjects of the Course. An intense program of lectures and seminars will be combined with supervised, hands-on practical training modules and group assignments using experimental setups and tools provided by the world-leading research environment of the Bordeaux School of Neuroscience.

Course director & co-directors

  • Dmitri Rusakov (London’s Global University, UK)
  • Tatiana Korotkova (Max Planck Institute for Metabolism Research, Germany)
  • Grégory Giannone (Bordeaux Neurocampus, France)

Elena Avignone (Bordeaux Neurocampus, France)
Stephane Bancelin (Bordeaux Neurocampus, France)
Francisco de los Santos (Max Planck Institute Cologne, Germany)
Janosch Heller (FutureNeuro & Royal College of Surgeons, Ireland)
Tom Jensen (University College London, UK)
Ani Jose (Bordeaux Neurocampus, France)
Olga Kopach (University College London, UK)
Francois  Maingret (Bordeaux Neurocampus, France)
Philipe R. F. Mendonça (University College London, UK)
Luc Mercier (Bordeaux Neurocampus, France)
Dragomir Milovanovic (Charité University Clinic, Germany)
Petr Unichenko (University College London, UK)
James Reynolds (University College London, UK)
Olga Tiurikova (University College London, UK)
Petr Unichenko (University of Bonn, Germany)
Hanna Van Den Munkhof (Max Planck Institute Cologne, Germany)
Kaiyu Zheng (University College London, UK)

Students will learn:

  • Super-resolved organisation of the synaptic cleft
  • Nanoscale monitoring and probing of NMDA receptors
  • Super-Resolution probing of the axonal initial segment
  • RESOLFT nanoscopy
  • Super-Resolution Imaging of Brain Nano-Structure In Vivo
  • Nanoscale imaging and manipulation of synapse function to study the brain
  • Monitoring molecular machinery of synaptic plasticity
  • Probing the molecular basis of synaptic transmission
  • Optical sensors for neurotransmitters
  • Multiplexed imaging of presynaptic function
  • Ca2+ regulation of synaptic vesicle fusion in central synapses
  • Optical monitoring of volume transmission
  • Studying astrocytes in synaptic function with 1D-to-3D two-photon Ca2+ imaging
  • Project 1: Imaging prefrontal cortex neurons encoding social information
  • Project 2: Imaging hippocampal place cells in freely moving animals
  • Project 3: FRET imaging of NMDA receptor co-agonists dynamics
  • Project 4: Assessing the mechanosensitive properties of the membrane periodic skeleton (MPS) in neurons
  • Project 5: RESOLFT nanoscopy to study the fast reorganization of dendritic spines
  • Project 6: Single synapse imaging of glutamate release and pre-synaptic Ca2+ for study of pre-synaptic receptor function and short-term synaptic plasticity
  • Project 7: Quantitative intracellular calcium measurements using fluorescent lifetime imaging microscopy
  • Project 8: A viral strategy for targeting and manipulating principal neurons: probing the glutamatergic synapse function at multi-synapse imaging approach
  • Project 9: Probing of astroglial Ca2+ dynamics in organised brain tissue through fluorescent intensity and lifetime measurements
  • Project 10: Multicolor multiphoton imaging of single synaptic release sites in vivo
  • Project 11: High spatial-temporal imaging of a heterogeneous population of synapses in neuronal cultures using the iGluSnFR probe
  • Project 12: Super-resolution shadow imaging in the mouse brain
  • Project 13: Dual color single particle tracking to study membrane receptors dynamics
  • Project 14: Imaging tripartite synapses using super-resolution microscopy
  • Project 15: Nanoscale organization and dynamics of synapsin condensates

Keynote speakers

Thomas Blanpied (University of Maryland School of Medicine, USA)
Daniel Choquet (Bordeaux Neurocampus, France)
Rosa Cossart (Aix-Marseille University, France)
Olga Garaschuk (University of Tuebingen, Germany)
Laurent Groc (Bordeaux Neurocampus, France)
Michael Haüsser (University College London, UK)
Christian Henneberger (University of Bonn, Germany)
Christophe Leterrier (Aix-Marseille University, France)
Valentin Nägerl (Bordeaux Neurocampus, France)
Timothy A. Ryan (Weill Cornell Medical College, USA)
Ilaria Testa (KTH Royal Institute of Technology, Sweden)
Andrea Volterra (University of Lausanne, Switzerland)
Kirill Volynski (University College London, UK)

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