Advanced Imaging Methods for Cellular Neuroscience:
The students will obtain with this course hands-on experience with innovative techniques expected to be central in cellular neuroscience in the coming decade. They will include in vitro and in vivo gene transfer, cell and tissue imaging by confocal imaging and light sheet microscopy, photomanipulation in living tissue and optophysiology, patch clamp electrophysiology, imaging of proteins and lipids by super-resolution microscopy (STED and PALM/STORM), single-particle tracking methodologies, correlative light electron microscopy (CLEM), live imaging of protein interactions (FRET, FLIM). Rodent and human model systems (iPSCs, organoids) are central, and successful invertebrate models such as Drosophila and C. elegans will be also available.
Course directors:
- Volker Haucke, Leibniz Institute for Molecular Pharmacology (FMP), Berlin, Germany
- Britta Eickholt, Charité – Universitätsmedizin Berlin, Germany
- David Perrais, IINS, CNRS – University of Bordeaux, France
Confirmed Speakers:
- Vivian Budnik, University of Massachusetts Medical School, USA
- Pietro De Camilli, Yale School of Medicine, USA
- Mike Fainzilber, Weizmann Institute of Science, Israel
- Erika Holzbaur, University of Pennsylvania, PA, USA
- Erik Jorgensen, University of Utah, Salt Lake City, USA
- Gary Lewin, Max Delbrück Center for Molecular Medicine, Germany
- Klaus Nave, Max Planck Institute, Germany
- Silvio O. Rizzoli, University of Göttingen Medical Center, Germany
- Frédéric Saudou, Grenoble University Hospital CHUGA, France
- Gipi Schiavo, University College London, UK
- Stephan Sigrist, Freie Universitat, Berlin, Germany
- Patrik Verstreken, VIB-KU Leuven Center for Brain & Disease Research, Belgium